Table 1. Kinetic parameters of mutant and wild-type forms of FBP1 and FBP2.
| kcat [s−1] a | ks[μM], F-1,6-BP a | kis[μM], F-1,6-BP a | n, F-1,6-BP a | A50[mM], Mg2+ | n, Mg2+ | IC50, AMP [μM] a | n, AMP a | |
|---|---|---|---|---|---|---|---|---|
| FBP2 WT | 20.9±2.52 | 3.66±0.07 | 111±2.3 | 1.61±0.04 | 0.88±0.05 | 1.96±0.21 | 0.59±0.01 | 2.04±0.05 |
| FBP2 D187L | 15.3±0.56 | 12.9±0.64 | 120±12 | 1.72±0.05 | 3.22±0.23 | 2.05±0.27 | 1.44±0.06 | 2.29±0.21 |
| FBP2 L190G | 23.8±3.19 | 5.82±0.20 | 119±3.6 | 1.99±0.06 | 1.96±0.09 | 1.94±0.17 | 84.4±6.7323300±2520 | 2.06±0.331.68±0.25 |
| FBP1 b | 16.7±0.5 | 2.2±0.4 | 157±48 | 1 | 0.31±0.01 | 1.93±0.12 | 5.06±0.27 | 1.95±0.16 |
Data presented as mean±SD
a – determined at Mg2+ concentration of 5 mM for wild-type FBP2, 15 mM for D187L mutant and 10 mM for L190G mutant.
b – as described in [20].