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. 2017 Dec 14;8(70):115526–115545. doi: 10.18632/oncotarget.23308

Figure 5. Effect of celecoxib and curcumol on cell cycle distribution.

Figure 5

(A) A549 and H1299 were treated with celecoxib (30 μM) and/or curcumol (30 μM) for 24 h. The cells were then fixed with 70% ethanol and stained with PI. The cell cycle distribution (G0/G1, G2/M and S) was determined by flow cytometry. (B) Western blotting was also performed to detect the levels of cell cycle regulators (cyclin D1, cyclin E, cdk2 and p21). Band intensity was quantified by Image J software. The results shown are representative of three different experiments. Data are represented as mean ± SD, *p < 0.05, **p < 0.01.