Table 1. The setup of next-generation metabolomics platforms.

Items	Next generation platforms
Sample types	Any biological fluids and tissues
Sample size	≥ 50 μl for liquid samples and 20 mg for tissues
Equipment	LC coupled with Quadruple, Ion trap, TOF, Qrbitrap
Solvent needed	Methanol, acetonitrile, IPA,
Modifiers	Formic acid, ammonium hydroxide, ammonium acetate, ammonium bicarbonate, ammonium carbonate
Columns	HILIC, reverse phase, normal phase
Data analysis software	Metaboanalyst; MSEA; Metlin; BioStatFlow; HMDB

Abbreviations: liquid chromatography(LC), time-of-flight(TOF), isopropyl alcohol(IPA), hydrophilic interaction chromatography(HILIC), metabolite set enrichment analysis(MSEA), human metabolome databse(HMDB).