Figure 5. Ribosomes accumulate in the C-terminus and over truncated stop codons in pelo-1.

(A) Metagene plot for all genes (solid lines), focusing on the C-terminus. Reads were assigned according to where their 5’ends map. Endogenous SKI/PELO targets (Figure 4A) are also shown (dotted line). Inset shows zoom of region just upstream of stop codons. (B) Metacodon plots showing where 15-18nt Ribo-seq reads terminate relative to nucleotides of each codon. For each nucleotide of each codon, we counted the number of times a 15-18nt Ribo-seq read terminated at that position (3’ end), and normalized to positions upstream of the codon (see Materials and methods). Sense codons of similar nucleotide composition are shown as controls, and all codons in Figure 5—figure supplement 1.

Figure 5—figure supplement 1. Metacodon plot of 3’end locations for all codons.

See Figure 5 for description.