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. 2017 Dec 12;6:e32027. doi: 10.7554/eLife.32027

Figure 4. Labeling of one uniPN and one multiPN connected to glomerulus VC1 with nSyb::CD19 and CD19::sdc.

Figure 4.

Expression of nSyb::CD19 (homozygote, top panels) and CD19::sdc (heterozygote, middle panels) in VC1 glomerulus induced GFP expression in a single uniPN (white arrow) and one multiPN (yellow arrow). With the nSyb::CD19 ligand (a), both the cell body of the uniPN (white arrow) and its dendrite branching in the VC1 glomerulus (white arrowhead) are visible. In contrast, the cell body of the multiPN is indicated by a yellow arrow. With the CD19::sdc ligand (b) the induction of GFP in the uniPN is comparable to that observed with nSyb::CD19 (white arrow and arrowhead point to the cell body and dendrite of uniPN, respectively). However, with CD19::sdc induction in the multiPN is stronger than with nSyb::CD19, and GFP labels the cell body (yellow arrow), its dendrites (yellow arrowhead), and its axon (pink arrowhead). High magnification image of the axon (white stippled inset) is shown in (d). (c) The diagram illustrates the position of the cell body of the multiPN with respect to the antennal lobe, and the trajectory of its axon (red stippled inset), projecting first into the LH (grey stippled circle), and then to the rostral part of the brain. This multiPN has been described previously - see (Marin et al., 2002 panel H, Figure 3). All images are maximum projection confocal stacks. Scale bar = 50 µm.