Table 1. Summary of the induction results in the identified glomeruli and circadian neurons.
(a and b) Summary of PNs labeled from ORNs expressing nSyb::CD19 (a) or CD19::Sdc (b) in identified glomeruli using the R17H02 and R28H10 LexA drivers. # of GFP+ PNs: number of PNs with GFP induction (median ±sd). % of positive ALs: percentage of antennal lobes with GFP+ PNs in identified glomeruli. # of PNs reported: numbers of PNs innervating identified glomeruli as reported in previous works (Grabe et al., 2016). In the column of R17H02, only the results from females (F) are listed because expression of the R17H02-lexA driver was highly variable in males. n: number of antennal lobes analyzed. *: one R17H02>nSyb::CD19 and two R17H02>CD19::Sdc VA6 glomeruli were GFP+ (there were GFP+ dendrites branching in VA6), but were excluded because the cell bodies of the uniPN projecting into VA6 could not be identified. $: with nSyb::CD19 there is GFP induction in multiPNs in animals homozygous for the ligand (hom), but not in heterozygotes (het). (c) Summary of neurons with GFP expression in animals with donor PDF neurons expressing nSyb::CD19. # of GFP+ Ns: number of the different types of neurons labeled with GFP (mean ±s.e.m). # of Ns reported with connections: previous works did not report these data. % of positive hemispheres: percentage of hemispheres analyzed that contained GFP+ neurons. n: numbers of the hemispheres analyzed.
| A | GH146-nlgSNTG4 + LexAop-nSyb::CD19 | ||||
| PNs | # of GFP+ PNs | # of PNs reported | % of positive ALs | n | |
| R17H02 | uniPN->DAl | 10.5±1.65 | 8±1.9(F) | 100 | 10 |
| uniPN->VA6 | 1±0.33 | 1±0.4 (F) | 100 | 9* | |
| uniPN->VA1lUm | 0 | 6±0.9 (F) | 0 | 10 | |
| multiPN | 0 (het)$
1±0.00 (horn) |
1 | 0 100 |
10 6 |
|
| R28H10 | uniPN->VCl | 1±0.00 | 1-2 | 100 | 22 |
| multiPN | 0 (het)$
1±0.48(hom) |
1 | 0 70 | 22 10 |
|
| B | GH146-nlgSNTG4 + LexAop-CD19::Sdc | ||||
| PNs | # of GFP+ PNs | # of PNs reported | % of positive ALs | n | |
| R17H02 | uniPN->DAl | 11.5±11.43 | 8±+1.9 (F) | 100 | 10 |
| uniPN->VA6 | 1±0.35 | 1±10.4 (F) | 100 | 8* | |
| uniPN->VA1lm | 0 | 6±0.9 (F) | 0 | 10 | |
| multiPN | 1±0.52 | 1 | 60 | 10 | |
| R28H10 | uniPN->VCl | 1±0.31 | 1-2 | 90 | 20 |
| multiPN | 1±0.39 | 1 | 90 | 20 | |
| C | nSybE-nlgSNTG4 + LexAop-nSyb::CD19 | ||||
| types of neurons | #of GFP+Ns | # of Ns reported with connections | % of positive hemispheres | n | |
| DN2 | 1±0.75 | N.A. | 55.6 | 18 | |
| DN3 | 7±2.3 | N.A. | 100 | 18 | |
| PER- | 7±2.81 | N.A. | 100 | 18 | |