| Voltage (kv) |
300 |
| Magnification |
290,000x |
| Defocus (μm, nominal) |
−1.0 to −2.5 |
| Pixel size (Å) |
1.02 |
| electron dose rate (counts/pixel/s) |
10 |
| Total electron dose (e- /Å2) |
80 |
| Exposure time (s) |
8 |
| Number of images (collected/processed) |
3900/3024 |
| Number of frames per image |
40 |
| Initial particle number |
1,843,269 |
| Particle number for 3D classification |
1,267,674 |
| Final particle for refinement |
937,118 |
| Resolution (masked/unmasked) (Å) |
4.0/4.2 |
| Map sharpened b-factor (Å2) |
−233 |
| Model refinement |
| r.m.s. deviation (bonds) |
0.005 |
| r.m.s. deviation (angles) |
0.97 |
| All-atom clashscore |
2.30 |
| Ramachandran plot |
| Outliers (%) |
0.00 |
| Allowed (%) |
4.59 |
| Favored (%) |
95.81 |
| CaBLAM analysis: |
| Outliers (%) |
1.92 |
| Disfavored (%) |
6.65 |
| Ca outliers (%) |
0.11 |
| Rotamer outliers (%) |
0.00 |
