Table 2.

PCR Primers for Mapping in Meiotic Progeny

	Marker	Temp in °C	Enzyme	Sizes in CC-124 (bp)	Sizes in CC-1952 (bp)	Primer Sequences
I	Chr1-0.3	53	XhoI	175	150, 25	5′CTA AGA AGC GCT TTG TGT GGG TCG A 5′CAT CCT TG CAA CAC TGT CCA TGC
	Chr1-4 (AGK)	53	NaeI	125, 75	200	5′ATT GTT GGG GCC TGG AAA TA 5′TGA ACG TAT GGT CCA GCG TA
	Chr1-7.55	53	BamHI	175	150, 25	5′AGG TTT GGG CTT TCC CCA ACC GCG G 5′GGA GCT ATG AGG GCT GAG GTT GTA
II	Chr2-1.7	53	–	600	700, 800	5′GGA GCT GGA CCT GTC CTA CA 5′GTA CAG GCC GGC ATT CTC
	Chr2-5.11 (GSP)	58	PstI	140	175	5′GGG GTA GGC GGG TAG CAG GCC TGC A 5′CAC AGC GCC TAC CAC AGT ATT CCT
III	Chr3-0.35	58	XbaI	100,125	225	5′GCT TGA GGG CGG TTT GGA GAG T 5′AGC AAT CGT GCG CAT TCC TCA G
	Chr3-1.1	53	–	300	200, 100	5′GTA TCC GGC TTG GTA CTG TTT GGA 5′AGG TGG ACC CCT CCG ACA AGG T
	Chr3-3.7 (DMAT)	48	–	300	250, 50	5′GGA CAT TCG TGT GGA GTG AA 5′GGG CAC GTC TGA CAG TAA CA
	Chr3-6.0 (TUA1)	53	MspI	150, 50	200	5′GGC CAT GAG TTG CTT CTT TC 5′AGG AAA CGC AGT CAA GGG TA
	Chr3-7.9 (PHOT)	51	DdeI	250, 75, 50	250, 125	5′TGC AGT TTT GCAGTT TGG AG 5′CTG CCG TCC ATG TTC CTT AT
IV	Chr4-0.5 (TUA2)	55	HaeIII	350	200, 150	5′CAC AAA CAC AAG TAC CGT TGG T 5′AAG GTC AGG GGG AAG ATC C
	Chr4-3.39	53	–	600	350	5′AGG GAG TAC GGC AAC ACC AG 5′GTC CCA AGT TCC TGC ATC C
V	Chr5-0.59	53	PstI	150	125, 25	5′AAT CCG CCA GCC GCT TCC CAG CTG C 5′GGT GCA CAA GGA GAT GAA CGT G
	Chr5-1.9 (ALDO)	53	–	300	700	5′GTT GGA GTT CCA TCG TCG TT 5′GTG GCT CCT CTC TGA ACT GC
	Chr5-2.56	53	–	450	>500	5′GTA GCT GTG AAA GGC CAA CG 5′GGT GAA TAC TAG CGG AGC ACA
	Chr5-3.1	51	–	Larger		5′TCC GTA CAG GTG GAG TAG GG 5′TTT AGG CCA CAC ACA CAT GG
VI	MTA/MTD	53		MTA: 187 MTD: 124	–	MTA: 5′TCT CCA TGG GGT GTA TCA TC 5′CGT TTC AGC TCA CCA ACC MTD: 5′TTT GGA GTC CTC TCG TCA AG 5′GCA TGG CCT CTT AAT CAG AC
	Chr6-3.41 (PP1)	51	MspI	150, 50	200	5′GCG ACG ATT ATG TTT GCT GA 5′GTG TCA CCC CGA TTC AGT TT
	Chr6-8.77	60	HindIII	75, 25	100	5′TCC AAC GAC TCG GCG CGT ACC ACA A 5′GCA TCT TCG TGG GCT ACC AGC A
VII	Chr7-0.5	57	HindIII	150, 100	250	5′CAT GTG GAG TGA TAT TTG TGA GCA TG 5′CAG TTA GCA GTT GGC CCA CGT CA
	Chr7-2.1	53	–	350	250	5′ACC TGC CTC CTT GGC ATC AAG C 5′GCG ACA AAT TGA TTG GTG GCT ATA A
	Chr7-6.1	53	PstI	200	175, 25	5′CTG CAC TTG TCA AAT TGG AGA TGC T 5′TAG CAA TAA GAC GGC CCA GAT GTT
VIII	Chr8-3.63	55	SalI	150 × 2	300	5′TCT CTC ACG CTC TCA CAC TCT TCC 5′GCG TTT GCT ACC AGA ACA AAA AGG
	Chr8-4.79	53	–	650	400	5′GCA TCA TGC GAG GTG TGT TA 5′ATC ATC ATC TGG CGC TTA GG
IX	Chr9 0.29	53	–	600	450	5′CCT CTA ACT CCG CCA GTG CT 5′GTC AAG ATG GAC CTG CTG CT
	Chr9-2.28 (TRX)	51	ScrFI	200	150, 50	5′TTC CGT CCT CAC CAA AGT TC 5′CCA GCA GAA TTG ACA GCA AA
	Chr9-3.24	51	HaeIII	Single	Double	5′TTT AGC TGA GGC GAG GT 5′AAA ATG GGA TGC GCA GTT AG
	Chr9-4.4	53	–	500	650	5′CGG GAA GAT AGA GGC GTT AG 5′AAT TAG GCG TGC TGG GTA AA
X	Chr10-0.3	53	–	400	275	5′GAA CGA GGC AGA GCT GAA CT 5′GGC GGC TAG GTA TGT GTG AT
	Chr10-1.71	53	–	300	350	5′AGT GCT CAT CTT CAG TAG CGG CTT 5′GTC CAT CAG GTA CGA CAG GGT TTC
	Ch10-3.65	53	EcoRI	185	160, 25	5′CTC ATT TGG GGG TTT TGC TAA CGA A 5′GTC AGC ACT AGT TAC GCA GGG GAG
	Chr10-3.82	53	PstI	175	100, 75	5′TAT TGG AGT GAT CGA GTG CCC TGC A 5′CGC CCA TGT AAC CAT AAG TCA
	Chr10-4.94	55	SalI	275	150, 125	5′CAG TTG CAC AAA CCG ACA AAG AAA 5′CTC GGT ACA CCC TGC CAT TCT G
XI	Chr11-0.5	53	–	400	250	5′GCT ATC GCA GGT TCC AGA AG 5′GAG CGG CCT ATT TCA GCA TA
	Chr11-3.59	50	MspI	200,175, 25	400	5′CCC CGA TTT CGA AGT TGA TA 5′ATA AGT GCG AGG GGG AGA CT
XII	Chr12-3.63	53	–	500	300	5′ACG TTG TTG AAG TGC AAT GG 5′CGG CGG ATG AAG ATG ACT
	Chr12-3.72 (LC3)	55	HaeIII	475	Larger	5′TGC ACA ACA CAA CAA GCA GA 5′ACA GGC GAG GAC ACA GCT AC
	Chr12-5.9	53	–	250	200	5′ACT AGA GGG CCG AGA CGG AAT GTA 5′CTG CTG GGC GTA CCT GTA AAT GTT
	Chr12-8.03 (LAO)	60	HaeIII	150, 75	225	5′TCT TAC AGC GAC CGG ATT TC 5′GAC ACC ACA TCAGGG CTA CA
XIII	Chr13-1.25	53	–	500	425	5′GAC AAT GTG CCA ACA GCA AG 5′AGG GAC TGG ACC AGC ACA G
	Chr13-2.09	56	PstI	225	150, 75	5′TAC CCC CTT CCT TGA ATC CAC TGA C 5′TCC ACA ACA CCA TAG TTA CCC CAA C
	Chr13-4.05	53	–	500	700	5′AGC AAT GGG TGG TGT TCA GT 5′AGG GAT CGT TCT AGG GCT GT
	Chr13-4.54	53	TseI	100, 50	175	5′CAC ACC CCA ATT CGT TGA GT 5′GCC TAT CCA GAC GCA AGC TA
XIV	Chr14-1.62	53	–	300	500	5′GAG AGC GAG TGC TTG GAA AT 5′GTC ATT GAG GCC GTG AAG AT
	Chr14-1.84 (ASB)	50	RsaI	300, 25	200, 100, 25	5′GGG CTA GGC CCT TTA CCT C 5′AGC TCC CGC ACT GTT TGT TGT AG
	Chr14-3.69	53	HindIII	200	175	5′AGT AAT GTA TGC ATC TTG CGG AAG C 5′TGT GAA GCG AGT CAG TAG AAA CCG
XV	Chr15-1.25	53	HindIII	50, 25	75	5′ TCG TTG AGC CAA AGC ATC CGC AAG C 5′CAG CAA CAT TGA GGC TAT CCT GGT
XVI	Chr16-0.61	53	–	175	225	5′CTG ACA AGG GTC AGA TGG AGG AGT 5′TGT ACG GCT GTT TTG AGT ACA CGG
	Chr16-3.04 (RAA2)	61	–	650	500	5′ATT GAC CAC TGC GGC GCT AGC G 5′TAG TAG GGC CAT CCG TGG CTC TCG
	Chr16-7.1	53	–	250	150	5′TGC AGA CTT GCA TTC GTA CAG AGG 5′CGT TGG AGC TGA CAG CAG TCC C
	Chr16-7.25	58	SalI	150, 100	200	5′GAG CCA GTG TGA GCC TTG TGT G 5′CAG TAA GGA TGG GAT GGG TGT GAG
XVII	Chr17-0.57H	53	HindIII	200	175, 25	5′GCA CAG CTG AAG CGCAAA AGG AAG C 5′CGT TTC TCG AAC TCA GCC ACT GT
	Chr17-0.57	53	–	175	200	5′TGC GAG GAG GTA TCG AAG AAA GAG 5′CCG TCT ACC GGC ACT ACG AGT C
	Chr17-2.23 (LC5)	53	MspI	150, 50	200	5′GGA CGG TGG GTA TGC ATT AG 5′GCT GTC ACT ACG TGG TCT CG
	Chr17-4.0	53	–	300	275	5′ATA TTA CGC CTC TCC GAC AAC AGC 5′CAG CTT CTT TGT GCG CTT GTA CTT
	Chr17-6.8	53	EcoRV	125	100, 25	5′CAT CGA GCT GCT TGG AGG CCA GAT A 5′CGC TAT ACA CCA CAT AGC GTC GAG