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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Microbiol. 2017 Dec 18;107(3):344–362. doi: 10.1111/mmi.13885

Figure 3.

Figure 3

Depletion of TbSmee1 leads to a slow-growth phenotype and causes a cytokinetic delay. (A) TbSmee1 cKO cells were grown for 8 days in either the presence (+; Control) or absence (-; TbSmee1 Removed) of doxycycline. Cells from each culture were monitored by cell count and collected daily to monitor for TbSmee1 depletion by Ty1 western blotting, using tubulin as a loading control. T0 represents the culture at the start of each experiment. (B) Cells expressing TbSmee1 (Control) and cells depleted of TbSmee1 (TbSmee1 Removed) for 6 days were fixed with PFA and treated with DAPI to label DNA. These cells were evaluated by fluorescence and DIC imaging. Asterisk shows a detached flagellum tip. (C) Quantification of DNA content of TbSmee1-depleted cells (TbSmee1 Removed) and control cells (Control) after 6 days. The “other” category contains a mixed population of multi-kinetoplast cells and nucleus-only cells. Data are means ± SD of three independent experiments.