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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Microbiol. 2017 Dec 18;107(3):344–362. doi: 10.1111/mmi.13885

Figure 4.

Figure 4

TbPLK localization is absent from the new FAZ tip when TbSmee1 is depleted. (A) Control cells (Control) and cells depleted of TbSmee1 for 6 days were fixed and labeled with antibodies against TbPLK (TbPLK; green) and FAZ (FAZ; red). DAPI was used to visualize DNA (DNA; blue) and the samples were imaged using fluorescence and DIC microscopy. Arrowheads denote PLK localization. (B) Quantitation of the localization of TbPLK in control and TbSmee1-depleted cells (TbSmee1 Removed) in A, sorted by DNA content. Data are means ± SD of three independent experiments.