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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Microbiol. 2017 Dec 18;107(3):344–362. doi: 10.1111/mmi.13885

Figure 6.

Figure 6

Depletion of TbSmee1 leads to altered hook complex morphologies. (A) Control cells and TbSmee1-depleted cells (TbSmee1 Removed) were fixed and labeled with an antibody against TbCentrin4 (TbCentrin4; green), antibodies against TbMORN1 (TbMORN1; red), and DAPI to visualize DNA (DNA; blue). A non-dividing 1N1K cell is shown. Insets are threefold magnifications. (B) Six-fold magnifications of TbMORN1-labeled (TbMORN; white) hook complexes with varying morphologies from non-dividing 1N1K control and TbSmee1-depleted cells (TbSmee1 Removed) categorized as shown in the schematic. (C) Quantification of hook complex morphologies in non-dividing 1N1K control and TbSmee1-depleted (TbSmee1 Removed) cells. Data are means ± SD of three independent experiments. *, P ≤ 0.05; ***, P≤ 0.001; ****, P ≤ 0.0001(multiple unpaired two-tailed Student’s t test).