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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: Mol Microbiol. 2017 Dec 22;107(3):428–444. doi: 10.1111/mmi.13892

Figure 6. The presence of VapA prevents the accumulation of LAMP1 within the RCV 48 and 72 h post infection.

Figure 6

(A) BMDMs were infected with either R. equi 103S or ΔvapA strains, and 100 nM rVapA32-189 was added the night before the infection, where indicated. At indicated time points, cells were fixed, immunostained, and visualized via confocal microscopy. R. equi (GFP, green), BMDM nucleus (DAPI, blue), VapA (anti-VapA, red), and murine LAMP1 (anti-LAMP1, purple) were observed. n = 3, bar = 5 μ, inset bar = 1 μ. (B) Representative confocal image of 103S infection of BMDMs at T72, performed and stained as in (A). A large RCV containing replicating bacteria and RCV membrane-associated VapA (arrow) is shown in comparison to macrophages containing strongly LAMP1-positive compartments surrounding bacteria that lack detectable VapA at the RCV membrane (asterisks), bar = 5 μ.