Table 1.

Transfection of de novo formed Sup35PrD-GFP oligomers from vps5Δ strains convert [psi^-] to [PSI⁺]. Freshly obtained lysates from cultures overexpressing Sup35PrD-GFP for 24 hours in either the [pin^-][psi^-] (which cannot induce [PSI⁺]) or [PIN⁺][psi] (which can induce [PSI⁺]) background were transfected into [psi-][PIN⁺] recipient cultures. Numbers indicate the percent of transfectants (approximately 130-250 transfectants scored) that were converted to [PSI⁺]. Induced vps5Δ cultures were also lysed and transfected at the same time as the WT controls. Experiments were performed as previously described (Sharma et al., 2017). Binomial comparison of conversion frequencies between WT [PIN⁺] and vps5Δ [PIN⁺] show that the two values are significantly different (p < 0.0001)

	No lysate	Donor lysates prepared after Sup35PrD-GFP overexpression for 24 hours
		WT [pin-]	WT [PIN+]	vps5Δ [PIN+]
Recipient wildtype [psi-] [PIN+]	0%	0.5%	28.7%	15.9%