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ITC parameters for Nfu binding to HSPA9 in the presence of 1 mM ADP or 1 mM AMPPNP at 25 °C.
| binding sites | KD (µM) | ΔH (kcal/mol) | ΔS (cal/mol K) | |
|---|---|---|---|---|
| Full-length Nfu/HSPA9 (ADP) | 0.9 ± 0.1 | 8.3 ± 1.4 | −6.3 ± 0.7 | 2.3 |
| Full-length Nfu / HSPA9 (AMPPNP) | 0.9 ± 0.2 | 36 ± 4 | −4.9 ± 0.1 | 3.7 |
| C-Nfu/HSPA9 (ADP) | 1.0 ± 0.2 | 18 ± 2 | −7.3 ± 1.3 | 2.8 |
| C-Nfu / HSPA9 (AMPPNP) | 1.0 ± 0.4 | 51 ± 6 | −3.2 ± 0.2 | 8.9 |
