Table 3.
ITC parameters for Nfu binding to Hsc20 at 25 °C.
| binding sites | KD (µM) | ΔH (kcal/mol) | ΔS (cal/mol K) | |
|---|---|---|---|---|
| Full-length Nfu/Hsc20 | 0.93 ± 0.06 | 10 ± 1 | −0.9 ± 0.1 | 20 |
| C-Nfu/Hsc20 | 0.94 ± 0.06 | 11 ± 2 | −1.7 ± 0.1 | 17.1 |
ITC parameters for Nfu binding to Hsc20 at 25 °C.
| binding sites | KD (µM) | ΔH (kcal/mol) | ΔS (cal/mol K) | |
|---|---|---|---|---|
| Full-length Nfu/Hsc20 | 0.93 ± 0.06 | 10 ± 1 | −0.9 ± 0.1 | 20 |
| C-Nfu/Hsc20 | 0.94 ± 0.06 | 11 ± 2 | −1.7 ± 0.1 | 17.1 |