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ITC parameters for C-Nfu binding to HSPA9, or Hsc20, in the presence of N-Nfu.
| binding sites | KD (µM) | ΔH (kcal/mol) |
ΔS (cal/mol K) |
|
|---|---|---|---|---|
| C-Nfu/N-Nfu/HSPA9 (ADP) | 0.88 ± 0.14 | 14.9 ± 1.67 | −3.82 ± 0.67 | 9.28 |
| C-Nfu/N-Nfu/HSPA9 (AMPPNP) | 0.907 ± 0.05 | 30.3 ± 2.82 | −2.00 ± 0.13 | 14.1 |
| C-Nfu/N-Nfu/Hsc20 | 0.79 ± 0.03 | 10.0 ± 1.22 | −0.57 ± 0.03 | 21.0 |
