Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 22;8:1326. doi: 10.1038/s41598-018-19640-3

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

TatE partly prevents premature cleavage of the TorA signal peptide. (A) The Tat substrate TorA-MalE335 (TMal) was synthesized and radioactively labeled in vitro and incubated with inverted inner membrane vesicles obtained from E. coli strain BL21(DE3)Δtat expressing the plasmid-encoded Tat components indicated. The samples were resolved by 10% SDS-PAGE and analyzed by phosphorimaging. In the presence of TatABC-containing vesicles, cleavage of the precursor (pTMal) to the mature protein (mTMal) is accompanied by the acquirement of proteinase K resistance (compare lanes 2 and 4) indicating transport into the lumen of the vesicles. Vesicles lacking TatB cleave pTMal (lane 5) but do not allow transport to occur (lane 6, no protease resistance). TatB (lane 7) and to a lesser extent TatE (lane 9) prevent this premature cleavage. (B) Quantification of premature processing of pTMal to mTMal by the indicated membrane vesicles was obtained from nine independent experiments using vesicles from two different preparations each, except for ΔTat vesicles (three experiments, one preparation).