Table 1.
Primer sequences of the cDNAs coding for the proteases receptors and proteases-related enzymes used for the real-time PCR.
| Gene | Primers: forward (F)/reverse (R) | ||
|---|---|---|---|
| Access number | Species | Name | |
| NM_004995.3 | Homo sapiens | Matrix metallopeptidase 14 (MMP14) | F: 5′GCTCTCTTCTGGATGCCCAA3′ R: 5′ GTACTCGCTATCCACTGCCC 3′ |
| NM_001908.4 | Homo sapiens | Cathepsin B (CTSB) | F: 5′TGCAATGTCACAACCTCTCTGA3′ R: 5′ TAGGAGGCAATCTGCAAACCA3′ |
| NM_001257973.1 | Homo sapiens | Cathepsin L (CTSL), transcript variant 5 | F: 5′CAAGTGGAAGGCTGCAATGG3′ R: 5′ AGTCCAGGCCTCCATTATCCT3′ |
| NM_001198868.1 | Homo sapiens | Calpain 1 (CAPN1) | F: 5′ CCATAGACATCTCCAGCGTTCT3′ R: 5′CACGTTGTTCCACTCTGAGGA 3′ |
| NM_001748.4 | Homo sapiens | Calpain 2 | F: 5′ CTCAACCAGGACTACGAGGC3′ R: 5′ TGATAAACTGGGGGTCAGCG 3′ |
| NM_001005377.2 | Homo sapiens | Plasminogen activator, urokinase receptor | F: 5′ACTCACGGACCGAAAAACCA3′ R: 5′TCCAGGTCTGGGTGGTTACA3′ |
| GAPDH | Homo sapiens | Glyceraldehyde 3-phosphate dehydrogenase | F: 5′CCTCCCGCTTCGCTCTCT3′ R: 5′GCTGGCGACGCAAAAGA3′ |