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. 2018 Jan 22;8:1373. doi: 10.1038/s41598-017-19086-z

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Simulated single vessel constrictions (a total of 25, with constriction cascade induced by constriction of SMA-covered vessels) and the subsequent changes in global tissue PO₂. Box plots indicated the percentage change in tissue PO₂, when compared to baseline tissue PO₂, as a consequence of singular constrictions of (a) penetrating arterioles, (b) precapillary arterioles and (c) capillaries. (d,e,f) 3-dimensional visualisations of spatial changes in tissue PO₂ as a result of constrictions in (d) a penetrating arteriole, (e) a precapillary arteriole and (f) a capillary. (g) 3-dimensional visualisation of the cortical network, in which black lines indicate the initial site of constriction for (d) and (e), and where the capillary constriction in (f) occurred downstream of (e). Note, absolute percentage changes < 20% for (d) and absolute values < 5% for (e) and (f) are not shown, for clarity.