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. 2018 Jan 22;8:1305. doi: 10.1038/s41598-018-19289-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Activation of the P2&3 TT-probe by S. aureus grown to different stages in TSB and RPMI. Left Y-axis and lines in black indicate CFU per mL, right Y-axis and lines in red indicate probe activation as a percentage of the maximum activation. All experiments were done in triplicate, and the plots present the average numbers of the measurements. Capped lines indicate standard deviation. Strains included were: A, S. aureus Newman wild-type; B, nuclease deficient S. aureus mutant (Δnuc); C, S. aureus clinical isolate X²⁹; and D, S. aureus clinical isolate U²⁹.