Table 2.
Quantification of cidA-cidB operon in different wPip genomes
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The number of copies of the cidA and cidB genes was estimated by real-time q-PCR, relative to the reference gene wsp known to be present as a single copy per genome. The number of copies was determined by calculating the ratio between cidA (or cidB) signals and wsp signals on four individuals per line. ± means s.d.The cidA to cidB ratio was always close to one. The numbers of cidA and cidB copies present per Wolbachia genome were therefore similar, as expected for an operon.