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. 2018 Jan 22;8:1376. doi: 10.1038/s41598-017-18865-y

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Schematic of phylogenetic relationships among different trypanosomatids employed in this study based on several works^15,42–48. Bodo saltans was represented as outgroup. Coloured boxes represent the presence of different myosin genes. Hashed boxes represent possible gene loss events. Gd – MyoG-derived gene. Hd – MyoH-derived gene. Numbers in circles near some nodes are references to the divergence time estimates in mya (millions years ago), as inferred by Lukes et al.¹⁵: 1 – 463-695 mya; 2 – 231-283 mya; 3 – 96-105 mya; 4 – 118-170 mya; 5 – 52-96 mya; 6 – 30-63 mya; 7 – 31-65 mya; 8 – 25-54 mya; 9 – 9-23 mya. The branching is not drawn to scale.