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. 2018 Jan 22;8:1323. doi: 10.1038/s41598-018-19643-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Expression of CCR5 by pancreatic cancer cells (A) AsPC-1; BxPC-3 and MIA PaCa-2 cells were stained with FITC-conjugated isotype or anti-CCR5 antibodies and quantified by flow cytometry. Histograms represent isotype and respective CCR5 fluorescent intensity. (B) Total protein (~30 µg) from AsPC-1, BxPC-3 and MIA PaCa-2 cell line was resolved by SDS PAGE gel. Expressions of CCR5 were detected by western blot analysis with anti-CCR5 antibodies. β-Actin antibody was used as a loading control for all the samples. (C) CCR5 expression levels in AsPC-1, BxPC-3 and MIA PaCa-2 cells, Immunostained with anti-CCR5 antibody and all the cells nuclei were counterstained with DAPI. Bars correspond to the mean ± S.D. Data are representative of three independent experiments.