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. 2018 Jan 22;8:1368. doi: 10.1038/s41598-018-19542-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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GGA2 colocalizes and interacts with EGFR in vivo. (a) ARPE-19 cells overexpressing GFP-tagged GGA2 were fixed and double immunofluorescence staining was performed using antibodies against EGFR (red) and TGN46, EEA1, or cathepsin D (blue). Arrows and arrowheads indicate colocalization of three signals in the TGN and peripheral structures, respectively. Bar, 20 μm. (b) ARPE-19 cells were fixed for triple immunofluorescence analyses using antibodies against Alexa488-EEA1 (green), endogenous GGA2 (red) and EGFR (blue). Arrowheads indicate colocalization of three signals. Bar, 20 μm. (c) ARPE-19 cells transfected with siCtrl, siGGA2, or siEGFR were processed for PLA to detect GGA2-EGFR or GGA2-CIMPR interaction (green). Nuclei were stained with DAPI (blue). Bars, 20 μm.