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. 2018 Jan 22;8:1366. doi: 10.1038/s41598-018-19200-9

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Validation of miRNA and mRNA sequencing results within the validation groupby q-PCR. Sequencing results were validated within the validation group of 30 normal glucose tolerant (NGT) and 30 gestational diabetes (GDM) pregnant women by q-PCR. (A) Six miRNAs were selected for q-PCR validation experiments within the validation. (B) Seven mRNAs, which are all predicted targets of miRNA-340 were selected for q-PCR validation experiments. Samples were paired based on maternal age and body mass index. Data was adjusted for pregnancy week and weight gain till the end of second trimester. Shown are the means ± SD. Differences considered as statistically significant (P ≤ 0.05) are marked by bold fonts.