Table 2.
Primers and product size of genes used for validation using quantitative real-time polymerase chain reaction.
| Gene symbol# | GenBank accession number | Forward (F) primers (5′-3′) | Product size (base pair) |
|---|---|---|---|
| Reverse (R) primers (5′-3′) | |||
| Hsp25 | NM_001010842 | F: CCGTCTTCTGCTGAGAGGAGTG | 117 |
| R: ACCGTTGTTCCGTCCCATCAC | |||
| Hsp90aa1 | NM_001109785 | F: GGTGTTGGTTCCTACTCTGCTTAC | 76 |
| R: ACTGCTCATCATCATTGTGCTTGG | |||
| Cirbp | NM_001031347 | F: GCCTGGGTACAAATTGGAAG | 72 |
| R: GCAGGTTGAACATACAAGCAAG | |||
| ApoB | NM_001044633 | F: TTCCAGCTTCCACGTATCCC | 181 |
| R: ATTTGGACGTTGCTTGAGCTG | |||
| Prdx4 | BX935739 | F: ACATGCACTTAGGGGCCTTTT | 90 |
| R: TCCACTGATCTCCCCACAGG | |||
| Cyp19a1 | NM_001001761 | F: AGACGGTGCAGAGTACAGAC | 92 |
| R: AGGCTGCCTTTCTATTGGGTG | |||
| Hsd17b1 | NM_204837 | F: CTCGGAGCAGGCCATGAGAG | 70 |
| R: GAAGGCCTGAATGGTGCGT | |||
| Serpinh1 | NM_205291 | F: AGCGCCCTGAAATCCATCAA | 171 |
| R: GAAGCCACGGTTATCCACCA | |||
| 18s rRNA | KT445934.2 | F: GGGATGCAGATCTTCGTGAAA | 117 |
| R: CAAAAGCTTGTGTCGAGGGC |
#Abbreviations: Hsp25, heat shock protein 25 gene; Hsp90aa1, heat shock protein 90 alpha gene; Cirbp, cold-inducible RNA-binding protein gene; ApoB, apolipoprotein B gene; Prdx4, peroxiredoxin 1 gene; Cyp19a1, cytochrome P450, family 19, subfamily A, polypeptide 1 gene; Hsd17b1, hydroxysteroid 17-beta dehydrogenase 1 gene; Serpinh1, heat shock protein 47 gene.