Fig. 6.

LPCAT2-induced LD accumulation blunts ecto-CRT exposure. a HT29 cells were treated for 48 h with or without FOX and then stained with Bodipy 493/503 (green) and CRT antibody (FMC75) conjugated with goat anti-mouse Alexa568 antibody (red) (upper panel). The Pearson correlation coefficient was calculated between CRT and Bodipy fluorescences (lower panel). b LDs were isolated from confluent cells treated or not with FOX for 48 h. A representative blot of compartment markers of LDs, total membrane (TM), cytosol (cyt) and post-nuclear supernatant (PNS) fractions is shown from three independent experiments. c Cell-surface CRT immunostaining after 24 and 48 h of chemotherapy treatments. Data represent CRT-positive cells in the DAPI-negative fraction. Data are mean ± s.e.m. of three independent experiments. P values were determined by the multiple Student t test. *p < 0.05, **p < 0.01, ***p < 0.001. Error bars denote s.e.m