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Focal adhesion-like complexes can be detected in T cells spreading on uniform fields of integrin ligand. Primary human T cells were stimulated on coverslips coated with OKT3 and VCAM-1 (A) or ICAM-1 (B,C) for 17 min, then fixed and stained for talin and paxillin. The inset in (B) shows focal adhesion-like structures in a different cell that was stimulated on OKT3 + ICAM-1 for 25 min. Scale bar = 10 µm; inset in B, 8 µm2.
