Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Jan 18;9:22. doi: 10.3389/fimmu.2018.00022

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2018 Das, Yang, Arifuzzaman, Kim, Kim, Jung, Lee and Chai.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Comparison of only lipopolysaccharide (LPS)-inducible and interferon-g (IFN-γ)-primed LPS-inducible transcriptional datasets. (A,B) Heat map representation of the transcripts that were uniquely upregulated in LPS-inducible and IFN-γ-primed LPS-inducible bone marrow-derived macrophages (BMDMs), respectively. (C) Gene set enrichment analysis (GSEA) results showing C5 gene ontology (GO) terms collections of the GSEA molecular signatures database (MSigDB) v6.1 in LPS-inducible and IFN-γ-primed LPS-inducible upregulated inflammatory genes in BMDMs. The top GO terms are ranked by the NES. (D,F) Heat map representation of the positive regulators of inflammatory transcripts [cytokines, chemokines, and interferon regulated genes (IRGs)] that were either down or upregulated in IFN-γ-primed LPS-inducible BMDMs compared with only LPS-inducible BMDMs, respectively. (E,G) Validation of IFN-γ-primed LPS-inducible down or upregulated genes compared with only LPS-inducible BMDMs, respectively. Gene expression was normalized to the glyceraldehyde-3-phosphate dehydrogenase transcript levels. **P < 0.001 compared with the control. The data represent three independent biological experiments.

Comparison of only lipopolysaccharide (LPS)-inducible and interferon-g (IFN-γ)-primed LPS-inducible transcriptional datasets. (A,B) Heat map representation of the transcripts that were uniquely upregulated in LPS-inducible and IFN-γ-primed LPS-inducible bone marrow-derived macrophages (BMDMs), respectively. (C) Gene set enrichment analysis (GSEA) results showing C5 gene ontology (GO) terms collections of the GSEA molecular signatures database (MSigDB) v6.1 in LPS-inducible and IFN-γ-primed LPS-inducible upregulated inflammatory genes in BMDMs. The top GO terms are ranked by the NES. (D,F) Heat map representation of the positive regulators of inflammatory transcripts [cytokines, chemokines, and interferon regulated genes (IRGs)] that were either down or upregulated in IFN-γ-primed LPS-inducible BMDMs compared with only LPS-inducible BMDMs, respectively. (E,G) Validation of IFN-γ-primed LPS-inducible down or upregulated genes compared with only LPS-inducible BMDMs, respectively. Gene expression was normalized to the glyceraldehyde-3-phosphate dehydrogenase transcript levels. **P < 0.001 compared with the control. The data represent three independent biological experiments.