Figure 4.

Time series and differences in transcription factor (TF) and transcription co-factor (TcoF) expression in lipopolysaccharide (LPS)-induced and interferon-g (IFN-γ)-primed LPS-induced bone marrow-derived macrophages (BMDMs). (A,B) Heat map representation depicting the expression of early, middle, and late upregulated TFs and TcoFs in IFN-γ-primed LPS-inducible BMDMs, respectively (P ≤ 0.01, and log₂-fold change ≥ 1.0). (C) Validation of early (NFkBIA), middle interferon regulatory factor-1 (IRF1), and late (IRF7) upregulated TFs in IFN-γ-primed LPS-inducible BMDMs. Gene expression was normalized to glyceraldehyde-3-phosphate dehydrogenase transcript levels. **P < 0.001 compared with the control. The data represent three independent biological experiments. (D,G) Heat map representation of the TFs and TcoFs that were uniquely upregulated in IFN-γ-primed LPS-inducible BMDMs, respectively. (E,H) Heat map representation of the TFs and TcoFs that were uniquely upregulated in LPS-inducible BMDMs, respectively. (F,I) Heat map representation of common TFs and TcoFs in LPS-inducible and IFN-γ-primed LPS-inducible BMDMs.