Fig. 3.

Temperature-dependent activity of MtLPMO9B and MtLPMO9D towards RAC. RAC (1.7 mg mL⁻¹) was incubated with a MtLPMO9B (3 mg g⁻¹ substrate) or b MtLPMO9D (1.5 mg g⁻¹ substrate) in the presence of ascorbic acid (diamonds) or 3-methylcatechol (squares). Samples were incubated at pH 5.0 for 8 h. Soluble fractions obtained from the incubation of RAC with either MtLPMO9B or MtLPMO9D were further incubated with β-glucosidase (1 U per sample) to yield glucose (not shown), gluconic acid, and cellobionic acid only