Table 1.

Reported rAAV vector yields on the articles cited in this mini-review

	Method/design	Vector yield	Reference
AdV-based system
	Ad-free, triple plasmid transfection	120ETU/cell	Matsushita et al. (1998)
	Transient transfection, pXX2, unconventional start codon to modulate Rep expression	1.2 × 103ETU/cell 9.4 × 105 vg/cell	Xiao et al. (1998)
	Rep/Cap Hela stable cell line + AdV	up to 36 IP/cell	Clark et al. (1995)
	Transient transfection, pSH3/pSH5 plasmids which combined AAV Rep, Cap, and AdV-helper genes	1.3 × 104 vg/cell 135 ETU/cell	Collaco et al. (1999)
	Transient transfection with pDG plasmid, MMLV regulate Rep expression	150 IP/cell	Grimm et al. (1998)
	Transient transfection, Mtrep-CMVcap plasmid, E4orf6-only plasmid	104 vg/cell 23 TU/cell	Allen et al. (2000)
	Rep/Cap stable cell line A549 + AdV	262 TU/cell	Gao et al. (2002)
	Rep/Cap stable cell line + AdV-Cre vector	1.3 × 105 vg/cell 1.7 × 103 ETU/cella	Qiao et al. (2002)
	Self-replicating Rep/Cap helper construct	2 × 109 IU/well	Li and Samulski (2005)
	Transient transfection, Rep/Cap split system	2.6 × 105 vg/cell 37.8 TU/cell	Emmerling et al. (2016)
HSV-based system
	Transient transfection, HSV-rc/d27 amplicon system	480 vg/cell	Conway et al. (1997)
	Infection with rHSV-rc strain on AAV-GFP-integrated cell line	480 ETU/cell	Conway et al. (1999)
	Infection with rHSV Rep/Cap and rHSV-GFP	1.5 × 105 vg/cell 6 × 103IP/cell	Hwang et al. (2003)
	Double infection with ICP27-deleted rHSV strains	40 TU/cell	Booth et al. (2004)
	Production of rAAV serotypes 1,2, and 9, by double infection with ICP27-deleted rHSV strains	> 1.3 × 105 vg/cell > 9 × 103 IP/cell	Kang et al. (2009)
	Production of rAAV serotypes 1,2,5 and 8, by using suspension-adapted BHK cells infected with rHSV strains	up to 1 × 105 vg/cell up to 1 × 104 IP/cell	Thomas et al. (2009)
	Suspension-adapted BHK cells infected with rHSV strains	> 5 × 104 vg/cell	Knop et al. (2011)
BV-based system
	Initial design, triple-BV system, Rep/Cap genes controlled by IE1/polh promoters	5 × 104 vg/cell 30 TU/cell	Urabe et al. (2002)
	Swapping of AAV genetic elements from different serotypes to improve production of rAAV5	6 × 104 vg/cell	Urabe et al. (2006)
	Intron-splicing mediated expression	1 × 1011 vg/mL	Chen (2008)
	Stable cell line, integration of Rep/Cap sequences + hr2-0.9 homologous regions + RBE sites	> 105 vg/cell	Aslanidi et al. (2009)
	Stable cell line producing rAAV serotypes 1–12	up to 5 × 105 vg/cell	Mietzsch et al. (2014)
Yeast expression system
	4-plasmid system, individual expression cassettes regulated by Gal1/Gal10 promoters	~ 108 vg/mL	Barajas et al. (2017)

vg vector genomes, ETU enhanced transducing unit, TU transducing unit, IP infectious particle, IU infectious unit

^aAssuming 5 × 10⁶ cells per 10 cm plate were used for assay