Fig. 1.

Scheme of psbQ’ replacement by cat gene and qPCR assessment of deleted or introduced gene in ΔpsbQ’1 and ΔpsbQ’2 mutants. The transformation vector (32,361 bp, a) was introduced to cells aiming a double homologues recombination, according to the scheme. The PCR experiment assessed the presence of selected markers (a and b): q, psbQ’; p, ori; d, DTA toxin; c, cat (chloramphenicol resistance gene); k, kanamycin resistance gene; e, eEF-1a control gene (b). The transformation vector was used as a control. The quantities of the psbQ’ (black bars) and cat (white bars) genes were expressed in ratios to the constitutive ef1α gene (c)