Figure 1.

Example UHPLC–UV chromatograms showing a cyanocobalamin peak (3.27 min) from a cyanocobalamin standard and immunoaffinity purified extracts of the barley malt matrix (BM) supplemented with Co or Co together with RF and NAM and fermented with Propionibacterium freudenreichii 256 or 266 (a). Example Q‐TOF–MS/MS spectra of the cyanocobalamin peak (3.27 min) from the standard (b) and fermented matrix supplemented with Co (c) and the peak at 3.16 min (Figure A) identified as pseudovitamin B12 in the Co‐supplemented fermented BM (d). Co, cobalt chloride; RF, riboflavin; NAM, nicotinamide; M, cyanocobalamin