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. 2018 Jan 23;8:1964. doi: 10.3389/fimmu.2017.01964

Figure 3.

Figure 3

Messenger RNA (mRNA)-electroporated tolerance-inducing dendritic cells (tolDCs) maintained their capacity to induce T-cell hyporesponsiveness while stimulating IL-10 secretion in an allogeneic mixed leukocyte reaction. (A) IFN-γ levels in the supernatant of allogeneic peripheral blood lymphocytes (PBLs) cultured in the presence of control DCs or tolDCs were analyzed by an enzyme-linked immunosorbent assay. No differences in the level of secreted IFN-γ were observed in the supernatant of PBLs stimulated with nonelectroporated tolDCs as compared with that of mock- or C–C chemokine receptor 5 (CCR5) mRNA-electroporated tolDCs. (B) Levels of IL-10 in the supernatant of PBLs cocultured with control or tolDCs. Cocultures of PBLs with mock- or CCR5 mRNA-electroporated tolDCs contained higher levels of IL-10 as compared with cocultures of PBLs with the corresponding control DCs [mean ± SEM of four replicates (IFN-γ) or six replicates (IL-10)]. *p < 0.05, **p < 0.01, ***p < 0.001.