Table 1.

The semi-mature phenotype and tolerogenic messenger RNA (mRNA) expression profile of tolerance-inducing dendritic cells (TolDCs) was not affected by mRNA electroporation.

		Control DCs			TolDCs
		Non-EP	Mock EP	C–C chemokine receptor 5 (CCR5) EP	Non-EP	Mock EP	CCR5 EP
Protein expression
CD80	%	92.45 ± 1.69	87.97 ± 2.85	86.13 ± 3.92	20.18 ± 9.85***	16.45 ± 8.01***	16.37 ± 8.33***
	MFI	120.47 ± 12.09	81.15 ± 6.44+++	78.28 ± 6.81+++	40.60 ± 5.98***	39.35 ± 5.16***	38.23 ± 5.26***
CD83	%	90.95 ± 1.15	82.58 ± 2.22	82.55 ± 2.65	24.83 ± 7.56***	23.48 ± 6.58***	22.22 ± 6.90***
	MFI	29.50 ± 2.90	22.88 ± 3.01+++	23.55 ± 3.28++	16.48 ± 3.59***	15.67 ± 3.32***	15.65 ± 3.40***
CD86	%	99.33 ± 0.26	99.20 ± 0.30	99.10 ± 0.36	93.03 ± 2.01**	92.73 ± 2.05**	93.52 ± 2.13**
	MFI	762.33 ± 55.37	508.50 ± 82.98++	471.67 ± 8,797+++	212.65 ± 44.15***	165.17 ± 36.28***	183.30 ± 40.67***
HLA-DR	%	93.87 ± 5.85	92.95 ± 6.39	93.07 ± 6.43	88.82 ± 5.18*	88.02 ± 5.65	87.87 ± 5.64*
	MFI	337.00 ± 58.42	220.33 ± 25.92+	234.33 ± 21.40	97.05 ± 34.91***	75.95 ± 19.53**	75.07 ± 21.63**
mRNA expression
LILRB4	RNE	0.28570 ± 0.01755	0.29916 ± 0.00928	0.25645 ± 0.01017	1.90876 ± 0.11275***	1.95846 ± 0.196215***	1.96215 ± 0.2019***
TLR2	RNE	0.10107 ± 0.00992	0.11338 ± 0.00490	0.10679 ± 0.00676	1.74427 ± 0.15793***	1.95846 ± 0.22097***	2.04363 ± 0.27273***

Mean ± SEM of six replicates (protein expression) and three replicates (mRNA expression).

⁺Denotes a statistically significant difference from nonelectroporated DCs, within control DC or tolDC conditions.

*Denotes a statistically significant difference from the corresponding (i.e., non-EP, mock EP, or CCR5 EP) control DC conditions.

^*/+p < 0.05, ^**/++p < 0.01, ^***/+++p < 0.001.

MFI, mean fluorescence intensity; RNE, relative normalized expression.