Association of ΔΔCq value regulation of miR-196a-5p expression with (A) overall survival, (B) local recurrence-free survival, (C) lymph node metastasis-free survival, (D) distant metastasis-free survival, and (E) disease-free survival. Kaplan-Meier analysis (cut-off: The median ΔΔCq value) indicated that patients with relatively low ΔΔCq value regulation levels of miR-196a-5p experienced significantly shorter lymph node metastasis-free survival than those with high ΔΔCq value regulation levels (P=0.0079, log-rank test). Overall survival, local recurrence-free survival, distant metastasis-free survival, and disease-free survival showed no significant differences between low and high groups of miR-196a-5p regulations (P=0.6205, P=0.0502, P=0.5382, and P=0.1049, respectively, log-rank test).