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. Author manuscript; available in PMC: 2018 Jan 23.
Published in final edited form as: Stem Cell Res. 2015 Feb 7;14(3):323–338. doi: 10.1016/j.scr.2015.01.009

Table 1.

Number of proteins (protein groups) identified and quantified in five different cell lines (hESC (H9), hiPSCs (SB5-MP1 and iNC-01), fibroblasts, and PBMC) by ESI-MSe.

Cell line hESC–H9 hiPSC (fibro)–
SB5–MP1		 Fibroblasts hiPSC
(CD34+cells)
–iNC–01		 PBMC
Passage P34 P48 P22 P28 N/A P36 P53 N/A
# of proteins identifieda 1817 1624 954 1981 642 1660 1189 1522 939 1021
# of proteins quantifiedb 1690 1439 848 1785 547 1464 1074 1345 775 869
# of proteins selected for quantitative comparisonc 1510 1185 736 1448 320 1266 895 1104 587 691
# of proteins up–regulatedd 62 in H9 vs. SB5–MP1; 429 in SB5–MP1 vs. fibroblasts 540 in iNC–01 vs. PBMC
149 in H9 vs. iNC–01
# of proteins down–regulatedd 50 in H9 vs. SB5–MP1; 89 in SB5–MP1 vs. fibroblasts 242 in iNC–01 vs. PBMC
134 in H9 vs. iNC–01
a

Number of proteins confidently identified in at least two out of three technical replicates (sample injections).

b

Number of proteins depicted in the first row and identified by at least three peptides that are required for quantification.

c

Number of proteins depicted in the second row with CV ≤ 30% between at least two out of three technical replicates.

d

Proteins differentially expressed in both biological replicates (overlapped between both passages); N/A – not applicable; number of proteins used for quantitative comparison is shown in red.