FIG 7.

P66 does not confer cathelicidin resistance. B. burgdorferi cultures were grown to a density between 5 × 10⁷ and 3 × 10⁸ cells ml⁻¹ and then incubated with increasing concentrations of mCRAMP at 33°C for 3 h. Viable bacteria were enumerated by plating. Vehicle control replicated the ratio of 0.01% acetic acid plus 0.2% BSA to MHB, as found under the 200-μg ml⁻¹ mCRAMP condition. Dose-dependent killing of E. coli was determined in parallel. Percent survival was calculated by comparing each mCRAMP concentration to the 0-μg ml⁻¹ mCRAMP condition for each bacterial strain. Two-way ANOVA with Bonferroni's posttest was used to compare survival of B. burgdorferi strains incubated at different mCRAMP concentrations (*, P < 0.05).