TABLE 3.
Expression of CD4γ13 differentiation markers and transcription factors
| Lineage and differentiation markers | Mean CD4γ13 T cell clone mRNA signal (AU) |
Scorea | Gene product | |
|---|---|---|---|---|
| sBT13-7 | sBT16-8 | |||
| Lineage | ||||
| Cd4 T cells | 7,651 | 9,484 | ++++ | CD4 antigen |
| Cd8 T cells | 51 | 56 | 0 | CD8 antigen, beta chain 1 |
| NK/NKT cells | 82 | 27 | 0 | Killer cell lectin-like receptor subfamily B mem 1C |
| B cells | 40 | 41 | 0 | CD79A antigen (immunoglobulin-associated alpha) |
| Cytokine polarization | ||||
| Th1 | 523 | 402 | + | T-box 21 (T-bet) |
| Th2 | 1,677 | 4,184 | ++/+++ | GATA binding protein 3 (Gata3) |
| Th17 | 62 | 45 | 0 | RAR-related orphan receptor gamma (RORγT) |
| Th22 | 283 | 96 | +/0 | Aryl-hydrocarbon receptor (Ahr) |
| ? | 298 | 855 | + | Endothelial PAS domain protein 1 (Epas1) |
| ? | 1,456 | 3,741 | ++/+++ | Eomesodermin (Eomes) |
| Trm | ||||
| Klrg1 | 64 | 57 | 0 | Killer cell lectin-like receptor subfamily G, mem 1 |
| Klf2 | 113 | 88 | 0 | Kruppel-like factor 2 (lung) |
| Hnf1a | 90 | 102 | 0 | HNF1 homeobox A |
| S1pr1 | 90 | 61 | 0 | Sphingosine-1-phosphate receptor 1 |
| Ccr7 | 252 | 268 | + | Chemokine (C-C motif) receptor 7 |
| Zfp683 | 52 | 71 | 0 | Zinc finger protein 683 (Hobit) |
| Prdm1 | 1,121 | 772 | ++/+ | PR domain containing 1, (Blimp-1) |
| Rgs1 | 2,318 | 2,972 | ++/+++ | Regulator of G-protein signaling 1 |
| Rgs2 | 1,032 | 2,238 | ++ | Regulator of G-protein signaling 2 |
| Cd69 | 3,526 | 3,169 | +++ | CD69 antigen |
| Cd44 | 5,222 | 4,794 | +++ | CD44 antigen |
| Itgal | 5,688 | 6,527 | +++ | Integrin alpha L |
| Itga4 | 1,605 | 214 | ++/+ | Integrin alpha 4 |
| Itgb7 | 3,184 | 2,423 | +++ | Integrin beta 7 |
| Itga1 | 1,021 | 701 | ++/+ | Integrin alpha 1 |
| Itgae | 265 | 784 | + | Integrin alpha E, epithelial associated |
| Treg | ||||
| Cd27 | 25 | 23 | 0 | CD27 antigen |
| Il2ra | 1,070 | 1,285 | ++ | IL-2 receptor, alpha chain |
| Foxp3 | 108 | 136 | 0 | Forkhead box P3 |
| Ctla4 | 2,127 | 1,519 | ++ | Cytotoxic T-lymphocyte-associated protein 4 |
| Th22 | ||||
| Ahr | 283 | 96 | +/0 | Aryl-hydrocarbon receptor |
| Foxo4 | 179 | 156 | +/+ | Forkhead box O4 |
| Bnc2 | 43 | 39 | 0 | Basonuclin 2 |
a
Scores are defined as follows: <150 arbitrary units (AU [∼3× the CD8b signal]) = 0, 150 to 999 AU = +, 1,000 to 2,499 AU = ++, 2,500 to 7,499 AU = +++, and 7,500 to 10,000 AU = ++++.