FIG 2.

Zn starvation stress and growth inhibition mediated by CP. (A and B) Growth of C. albicans SC5314 was carried out and monitored as described for Fig. 1A with 500 μg ml⁻¹ CP (20 μM) in YPD medium supplemented with the indicated concentrations of ZnSO₄ (A) or 40 μM ZnSO₄ or MnSO₄ (B). Error bars are standard errors from two to four biological replicates. (C) ZRT1 and PRA1 mRNA was analyzed as described for Fig. 1D in C. albicans SC5314 grown with 500 μg ml⁻¹ CP and the indicated concentration of ZnSO₄. Error bars are standard errors for biological duplicates representative of two experimental trials; Zn-treated samples were compared to no-Zn, +CP samples for statistical analysis. (D) C. albicans strain SN152 (ZAP1⁺) or the isogenic zap1Δ/Δ strain was grown, and data were plotted as described for Fig. 1A. Error bars are standard errors for biological duplicates representative of two experimental trials. Significance was assessed using a two-tailed t test. ****, P < 0.0001; ***, P < 0.0003; **, P < 0.005; *, P < 0.05.