FIG 4.
GroEL2 proteolysis modulates DC antigen presentation and T cell polarization. (A) We pulsed DCs with various amounts of the OVA323–339 peptide (1 μg/ml, 10 μg/ml, and 50 μg/ml) for 6 h and then stimulated cells with either recombinant GroEL2 or GroEL2(cl) for 24 h. Following coculture with antigen-specific TCR-Tg CD4+ T cells for 72 h, we assayed cells for the cytokines IFN-γ, IL-2, and IL-17A by an ELISA. (B) We infected DCs with live M. tuberculosis strains and then cocultured them with purified ESAT-6-specific TCR-Tg CD4+ T cells. After 80 h, cell-free supernatants were collected and assessed for the cytokines IFN-γ, IL-2, and IL-17A by an ELISA. Data are shown as means ± SD of results of one representative experiment from three independent experiments.