Table 2. Cloning and Expression Vectors Used in This Study.

plasmid	description	reference
pRSF-Duet-1	Expression vector (KanR), T7 promoter	Novagen
pET-Duet-1	Expression vector (AmpR), T7 promoter	Novagen
pET-28b	Expression vector (KanR), T7 promoter	Novagen
pACYC-Duet-1	Expression vector (CmR), T7 promoter	Novagen
pME001	fadD gene with C-terminus His-tag from E. coli MG1655 cloned into pRSF-Duet-1 vector using the primers PrME001 and PrME002	This study
pME002	plsB gene with C-terminus His-tag from E. coli MG1655 cloned into pet-28b vector using the primers PrME003 and PrME004	This study
pME003	plsC gene with C-terminus His-tag from E. coli MG1655 cloned into pet-28b vector using the primers PrME005 and PrME006	This study
pSJ148	cdsA gene with N-terminus His-tag from E. E. coli MG1655 cloned into pACYC-Duet vector using the primers 103 and 106	Caforio et al.a
pAC004	pss gene with C-terminus His-tag from B. subtilis cloned into pACYC-Duet vector using the primers 89 and 90	Caforio et al.a
pAC008	psd gene with C-terminus His-tag from E. coli MG1655 cloned into pACYC-Duet vector using the primers 533 and 534	Caforio et al.a
pAC015	pgsA gene with C-terminus His-tag from E. coli MG1655 cloned into pRSF-Duet vector using the primers 551 and 552	Caforio et al.a
pAC017	pgpA gene with C-terminus His-tag from E. coli MG1655 cloned into pET-Duet vector using the primers 562 and 563	Caforio et al.a

^aRef (24).