Figure 6.

(A) ChIP qPCR showing that after treatment of Panc-1 cells with 1 mM H₂O₂ or 10 μM luteolin, the recruitment of OGG1 to the KRAS promoter increases more at G4 region than non-G4 regions (Ctr-1 and Ctr-2). Asterisk (*) indicate P < 0.05 (n = 4), a Student’s t-test was performed; (B) Primary sequences of 32R and designed 8-oxoG-substituted oligonucleotides where the positions of 8-oxoG are indicated; (C) The panel shows in a denaturing PAGE that OGG1 excises 8-oxoG in the duplexes formed by the designed 8-oxoG oligonucleotides and the complementary strand; (D) Sequencing 18% PAGE showing that OGG1 (1 μM) cleaves the radiolabeled duplexes (2 nM) exactly at the positions where there is 8-oxoG. The experiments in (C) and (D) were repeated three times.