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In vitro cap-dependent transcription assay. (A) Vmax of capped RNA primed RNA synthesis of A/H17N10 wild-type influenza polymerase (black) and PB2-variants R264A (pink), I260A (purple) and Y432A (orange) at varying concentrations of capped RNA primer (see Materials and Methods and Supplementary Figure S3). (B) Quantification of derived kinetic parameters for capped RNA primed transcription. Mean values and corresponding standard deviation of duplicate experiments are indicated.
