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fliFISH improves the resolution and reliability of counting RNA copies, especially when using a small number of probes. (A) Comparison between conventional smFISH (left image) and fliFISH (right image) using eight probes to target Ins2 mRNA in pancreatic β cells (MIN6) in culture. (B) fliFISH enables accurate localization of individual blinking events and the distinction between multiple transcripts within a diffraction-limited area.
