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. 2017 Oct 4;46(2):e7. doi: 10.1093/nar/gkx874

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© The Author(s) 2017. Published by Oxford University Press on behalf of Nucleic Acids Research.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 5. — fliFISH compatibility with multiplexed transcript quantifications in single cells. (A) Simultaneous detection of Ins2, Nkx2-2, and Gapdh mRNA copies, each targeted with 8 probes, tagged with Alexa 647, Atto 488 and TAMRA, respectively. Raw fluorescence images for each channel (left) and fliFISH image (right) of β cells in culture. (B) Detection of Ins2 by fliFISH using a two-color barcode, where probes tagged with Alexa 594 (left) or Alexa 649 (middle) were used simultaneously. The overlap of the two sets is shown on the right.