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. 2017 Dec 4;46(2):704–716. doi: 10.1093/nar/gkx1223

Figure 5.

Figure 5.

Ccq1 dependent negative regulation of telomere lengthening via Clr3-SHREC. (A) The yeast two-hybrid assay shows that Clr3 interacts strongly with Ccq1(500–735) and weakly with Ccq1(1–441). The indicated Ccq1 truncation proteins were fused to the GAL4 activation domain (AD) and Tpz1 and Clr3 were fused to the GAL4 DNA binding domain (BD). Selection plate lacks adenine and histidine. (B) Co-immunoprecipitation with the truncated Ccq1 showed that endogenously expressed Ccq1 associates with Clr3 via its C-terminus (amino acids 500–735). (C) Telomere Southern blot shows mild telomere elongation in clr4Δ, similar to ccq1(1–441). Deletion of clr3 and mit1 leads to elongation of telomeres only in the presence of the Ccq1-Est1 chimera.