Table 2. GFP::H2B, spo-11 & GFP::RPA-1, spo-11.
| Region | Hours post- microirradiation | Nuclei with RAD-51 foci clusters | Total number of RAD-51 foci clusters | Total numbers of microirradiated nuclei | Fraction of nuclei with foci clusters/microirradiated nuclei | RAD-51 foci clusters/nucleus with a cluster |
|---|---|---|---|---|---|---|
| PMT | 2 | 65 | 80 | 88 | 73.9 | 1.2 |
| PMT | 24 | 40 | 58 | 64 | 62.5 | 1.5 |
| PMT | 48 | 33 | 43 | 144 | 22.9 | 1.3 |
| PMT | 72 | 0 | 0 | 32 | 0.0 | NA |
| TZ | 2 | 36 | 49 | 90 | 40.0 | 1.4 |
| TZ | 24 | 44 | 71 | 70 | 62.9 | 1.6 |
| TZ | 48 | 7 | 8 | 80 | 8.8 | 1.1 |
| TZ | 72 | 0 | 0 | 40 | 0.0 | NA |
| MP | 2 | 57 | 71 | 96 | 59.4 | 1.2 |
| MP | 24 | 43 | 65 | 96 | 44.8 | 1.5 |
| MP | 48 | 0 | 0 | 80 | 0.0 | NA |
| MP | 72 | 0 | 0 | 56 | 0.0 | NA |
| LP | 2 | 64 | 76 | 78 | 82.1 | 1.2 |
| LP | 24 | 2 | 2 | 54 | 3.7 | 1.0 |
| LP | 48 | 0 | 0 | 30 | 0.0 | NA |
| LP | 72 | 0 | 0 | 24 | 0.0 | NA |
Number of RAD-51 nuclei with a foci cluster, total number of foci clusters, and total number of microirradiated nuclei per gonadal region for worms that were microirradiated and then dissected at 4 different time points post-microirradiation. Immunofluorescence was performed with an anti-RAD-51 antibody. ‘Fraction of nuclei with foci clusters/microirradiated nuclei’ indicates how many nuclei showed foci clusters out of the total number of nuclei that were microirradiated. ‘RAD-51 foci cluster/nucleus with a cluster’ indicates the average number of foci clusters per nucleus.