Table 3. Colocalization of RPA-1 and RAD-51 clusters.
| Numbers of foci | % of RPA-1 clusters | % of RAD-51 clusters | ||||||
|---|---|---|---|---|---|---|---|---|
| Region | Hours post-microirradiation | Mostly RPA-1 | Mostly RAD-51 | Colocalized | Mostly RPA-1 | With RAD-51 | Mostly RAD-51 | With RPA-1 |
| PMT | 2 | 6 | 13 | 33 | 15 | 85 | 28 | 72 |
| 24 | 2 | 20 | 31 | 6 | 94 | 39 | 61 | |
| 48 | 2 | 7 | 17 | 11 | 89 | 29 | 71 | |
| TZ | 2 | 15 | 12 | 12 | 56 | 44 | 50 | 50 |
| 24 | 5 | 3 | 42 | 11 | 89 | 7 | 93 | |
| MP | 2 | 16 | 16 | 10 | 62 | 38 | 62 | 38 |
| 24 | 6 | 11 | 21 | 22 | 78 | 34 | 66 | |
| LP | 2 | 2 | 11 | 43 | 4 | 96 | 20 | 80 |
Data indicating the hours post-microirradiation for 4 different stages, and subsequent measurements of RPA-1, RAD-51, or both colocalized foci in dissected, fixed, and stained worms. Immunofluorescence was performed by the combined use of both an anti-GFP antibody and an anti-RAD-51 antibody. The data is further divided into the percentage of RPA-1 clusters that were either present mostly alone or with RAD-51 cluster, as well as RAD-51 cluster that were present mostly alone or with RPA-1 cluster.